Ultrastructure of immature and mature human oocytes after cryotop vitrification
نویسندگان
چکیده
منابع مشابه
Ultrastructure of immature and mature human oocytes after cryotop vitrification
In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic...
متن کاملUltrastructure of human mature oocytes after vitrification
Since the introduction of human assisted reproduction, oocyte cryopreservation has been regarded as an attractive option to capitalize the reproductive potential of surplus oocytes and preserve female fertility. However, for two decades the endeavor to store oocytes has been limited by the not yet optimized methodologies, with the consequence of poor clinical outcome or of uncertain reproducibi...
متن کاملEffects of Vitrification on Outcomes of In Vivo-Mature, In Vitro-Mature and Immature Human Oocytes.
BACKGROUND/AIMS To observe the effects of vitrification on spindle, zona pellucida, embryonic aneuploidy and DNA injury in in vivo-maruted, in vitro-mature and immature human oocytes. METHODS Between January 2009 and February 2015, 223 immature oocytes from 450 infertile patients, and 31 in vivo-mature oocytes from 3 infertile couples were collected. Of the 223 immature oocytes, 113 were used...
متن کاملI-14: Vitrification of Human Immature Oocytes in IVM Program
The cryopreservation of immature GV oocytes by vitrification is offering attractive perspectives, since they represent an important pool of germ cells for women undergoing IVM cycles, or at risk of infertility for OHSS or PCOs, particularly those poor responders to gonadotropin stimulation, or in case of cancer. Therefore, it becomes necessary to optimize both cryopreservation technique as well...
متن کاملP-145: Maturation Capacity and Viability Assessment of Human Immature Oocytes After Vitrification and In Vitro Maturation (IVM)
Background: 15% of oocytes collected in ART cycles are immature. These oocytes may be matured following IVM program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM. The aim was to determine the maturation rate, morphology and viability of human immature oocytes after fresh IVM and vitrified-IVM program. Materials and Methods: 63 women who und...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2014
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.2014-027